In his office, U.S. Senator Stockwell is watching the broadcast of the launch of Star Flight XII with Central Intelligence Agency chief Victor Connelly when a mysterious male voice cuts into the television transmission. The man announces he has sabotaged the mission as punishment for Stockwell’s refusal to turn over microfilm containing nuclear missile plans, and seconds after liftoff, the spaceship blows up. Determined to catch the saboteur, Connelly feeds data about the event into the agency’s computers, which advise him to contact secret agent Sabrina Kincaid. After Stockwell and Connelly show Sabrina the carrier pigeon the saboteur had sent to pick up the microfilm, she contacts two members of her all-female spy unit, The Doll Squad, and recruits them for the assignment. Unknown to Sabrina, thug Munson and two cohorts have been following her and soon murder the recruits. Munson then tries to kidnap Sabrina, but she attacks him with a specially designed lighter that shoot flames into his face, then escapes unharmed. The next day, at a skeet shooting range, Sabrina tells Stockwell that a spy has infiltrated his office, and Connelly reveals that Munson’s thugs, who were apprehended, had silver disks implanted in their necks. After Connelly explains that implanted silver disks were part of another plot foiled three years before by a group of covert CIA agents, Sabrina secures the names of the agents, believing one of them may be the saboteur. Aided by computers, Sabrina identifies agent Eamon O’Reilly, a former lover who disappeared mysteriously after his last mission, as the likely traitor. Before she can alert Connelly, Nancy Malone, Stockwell’s secretary, tries to ambush her. The ever-alert Sabrina gets the drop on Nancy, who has been working for O’Reilly, and forces her to reveal that O’Reilly is living on Lorenzo, an island off the coast of Venezuela. Later, after Nancy kills herself with a cyanide pill, Sabrina assembles a new Doll Squad to track down O’Reilly. The karate-trained squad consists of “Cat,” a librarian; Lavelle Sumara, an exotic dancer; swimmer Sharon O’Connor; and psychologist Elizabeth “Liz” White. Meanwhile, at his compound on Lorenzo, O’Reilly, who learned about The Doll Squad from Nancy, plots with minions Maria and Joseph to waylay Kim Luval, a squad recruit working undercover at an island carnival. There, brutish Joseph overpowers Kim and delivers her to O’Reilly. O’Reilly then orders cohort Dr. Cahaymen to create a lifelike mask for Maria so she can impersonate Kim. Sabrina, meanwhile, checks into a Lorenzo hotel with her squad, and that night, goes to meet Kim at the carnival. Sabrina quickly deduces that the disguised Maria is not Kim and knocks her out. When Joseph tries to pursue Sabrina, who has taken the unconscious Maria prisoner, Cat blows up his car with explosives. Later, at the squad’s hotel room, Liz hypnotizes Maria and compels her to reveal the location of O’Reilly’s compound and Cahaymen’s house, where Kim is being held. The squad sneaks into the room where Kim is tied up and replaces her with the still hypnotized Maria. Soon after, O’Reilly orders a guard to kill Kim, and the guard unwittingly murders Maria. The next day, Sabrina, Lavelle, Kim and Sharon rent a boat to reach O’Reilly’s compound, while Cat and Liz rent a Jeep and head for the compound by land. Using the boat’s radio, Sabrina tries to hail Connelly, unaware that drug-addicted deck hand Rafael, an O’Reilly spy, has disabled it. Rafael then radios O’Reilly to alert him about the squad’s imminent arrival and, after the women take off in a skiff, follows them to shore. Cat and Liz, meanwhile, kill and disarm two guards protecting one of the compound’s perimeters by tricking them into drinking some alcohol laced with explosives. Nearby, Rafael catches up to Sabrina’s group and, while pretending to help them, leads them instead to O’Reilly’s guards. That night, after Sabrina, Kim, Sharon and Lavelle are handcuffed and imprisoned, Cat and Liz sneak up and begin firing on the men guarding them. Sabrina, Sharon and Lavelle are eventually freed, but Kim is shot and killed. As Cat and Lavelle plant explosives around the compound, Sabrina, Liz and Sharon steal weapons and attack the guards. In the ensuing melee, Sharon is wounded and Sabrina is captured and delivered to O'Reilly in the compound. O’Reilly explains to Sabrina that, with the help of Cahaymen and the doctor’s twin brother, he intends to release thousands of rats he has infected with bubonic plague in order to extort money from governments around the world. Still attracted to Sabrina, O’Reilly invites her to be his “queen,” but she rejects him. As Rafael leads her away, Sabrina grabs his gun and shoots him, then later eavesdrops on a meeting in which O’Reilly is addressing a group of spies representing the various countries he plans to target. Now fully aware of O’Reilly’s scheme, Sabrina arranges to meet her cohorts on the beach, after she has secured evidence from the compound. O’Reilly soon catches her, however, and takes her to his bedroom. There, the two enjoy a tense flirtation until Sabrina sprays O’Reilly with mace hidden in one of her rings and runs him through with the antique sword on his fireplace mantle. At the beach, Sabrina rendezvous with the squad, and together the women shoot and blow up the Cahaymen twins and O’Reilly’s remaining guards. The next morning, after the squad is picked up by the rental boat, Sabrina radios Connelly and demands a week’s vacation.